Streptococcus pneumoniae

Family_Web Streptococcus pneumoniae is associated with high morbidity in children, especially when related with the respiratory tract and otitis infections. The most severe complication of pneumococcal infection is invasive pneumococcal disease, including sepsis or meningitis. Although these forms of pneumococcal infection are relatively infrequent, in the neonatal period they are related with high morbidity and mortality.

Diagnosis of pneumococcal bacteremia typically relies on performing a blood culture, which is followed by gram staining, subculturing on sheep blood agar, and biochemical tests such as the bile solubility and the optochin susceptibility tests. The detection of pneumococcus may, however, take up to three days using the conventional methods. Furthermore, S. pneumoniae has a strong tendency of autolysis, which may significantly delay or hamper the diagnosis. Additionally,  issues remain in the differentiation between S. pneumoniae and the closely related viridans species

GenomEra® S. pneumoniae assay enables reliable and specific detection of pneumococcus from positive blood cultures or equivalent liquid bacterial cultures.

Clinical performance:

Sensitivity: 82/82= 100%
Specificity: 144/144 = 100%

Assay steps for blood cultures:

strepto_1Mix positive blood culture with buffer. strepto_2Pipette to test chips and start the GenomEra® CDX assay run.